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Allflasks linearscale sameaxes channel7.jpg

Successful Transformation ??!!?1!?!?!1?!!1

DVC EF oriC transformant vs WT on chlor.jpg

Seems to confirm that E. coli growth slows when carbohydrate concentration runs low?

2ndcoculture4.2carbs.png

CIRCUIT DESIGN google doc

Circuit parts google doc

BetterObviousCloningTimeline.png

TODO

Deliverables:

C. hutch Transformation Stuff

C. hutch Promoter/RBS Stuff

Write a Wikipedia article on C. hutch (at some point)

Orders:

Lab stuff

DNA stuff

Old Todo lists:

ADVISORS TODO

STUDENTS TODO

The Next Few Weeks

The Final Weeks

Schedule

08/14/15 Meeting

Jesse's Stuff for 8/22-8/29

Weekends

Saturday 7/25 & Sunday 7/26

Saturday 8/1 & Sunday 8/2

Saturday 8/8 & 8/9

Saturday 8/15 & Sunday 8/16

Saturday 8/22 & Sunday 8/23

Cloning

Restriction Digest Template

Resources

Protocols and recipes

Chemicals and equipment

Plasmids and Freezer Stocks

The Library (Papers and Resources)

Advisors' contact info

Cytophaga hutchinsonii Knowledge-Base

Meeting Notes

Experiment Template

Journal Club Template

Project Proposal and Presentation

Quote Book!

RESEARCH NOW

Quorum Sensing

InterLab

HPLC Knowledge-Base

List of Other iGEM Consortia Projects

Experiments

Initial Co-Culture Information (6-9-15)

Initial (Naive) Co-Culture Protocol (6-9-15)

CIDAR vs. NEB Transformation Test (6-9-15)

Growing E. coli on C. hutch. media (6-11-15)

Distinguishing E. coli and C. hutch from flow cytometer data

Growing C. Hutch on plates

Growing C. Hutch in liquid culture

Initial TLC FAEE Measurement Information

TLC Measurement of Pure/Initial FAEE Protocol

TLC Measurement of Carbohydrates

Culturing experiments

Making Biodiesel L1

OriC L0 vector

Biodiesel Producing Co-Culture (3rd Co-Culture)

Second Naive Co-Culture

Flow Cytometry Accuracy

InterLab

Testing Circuit Parts

Biodiesel Genes on Amp Backbone and Chlor Backbone

Growing E. coli on Dead C. hutch

A2A Biodiesel E. coli

Initial HPLC of FAEEs

RFP calibration for E Coli

08/13/15 Ayesha and Molly

08-19-15 Backbone cloning (Nick)

Checking A2A competent cells 8/21/2015

Ayesha - Cloning 8/26

Lab Notebooks

Team "Transform Hutchinsonii"

Team "Characterize Hutchinsonii"

Team "Make Biodiesel"

Dry Notebooks

Policy, Practices and Economics

Modelling

Circuitry

Science As Storytelling

Communications (Lab) Resources

Project Outline

Methods

Monsanto ppt

Poster

Poster google doc

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Biofuel System research

Social Media!

VIDEO

BIOS

Design TO DOs!

Questions Google Doc

About Us

Project Design

Team

Parts

Collaborations

Attributions

Today